ELECTROPHORETIC MOBILITY-IONIC STRENGTH STUDIES OF PROTEINS

Electrophoretic mobility-ionic strength studies of proteins. IV. The effect of lipids on the electrophoretic patterns of human serum albumin at acid pH.

In previous publications from this laboratory, we have investigated the anomalous behavior of human serum albumin (l), various animal albumins (2) and human -y-globulin (3) in the Tiselius electrophoresis apparatus at acid pH. Because of increasing interest in the behavior of proteins in the acid region below their isoelectric point, this phenomenon has been receiving increasing attention from ...

Diverging electrophoretic and dynamic mobility of model silica colloids at low ionic strength in ethanol.

Electroacoustics and laser Doppler electrophoresis were employed to measure the mobility of surface-modified silica colloids in ethanol as a function of the ionic strength. Sufficiently low volume fractions were chosen to exclude effects of interparticle interactions. At high ionic strength, the electrophoretic mobility μ(e) is equal to the (electroacoustic) dynamic mobility μ(d) at 3.3 MHz. Ho...

Ionic strength effects on electrophoretic focusing and separations.

We present a numerical and experimental study of the effects of ionic strength on electrophoretic focusing and separations. We review the development of ionic strength models for electrophoretic mobility and chemical activity and highlight their differences in the context of electrophoretic separation and focusing simulations. We couple a fast numerical solver for electrophoretic transport with...

Influence of protein concentration upon electrophoretic mobility of serum proteins.

Previous investigators have reported a change in distribution of serum protein fractions, as measured by the areas subtending the conventional electrophoresis curves, as a result of a change in ionic strength of the buffer (1) or as a result of a change in total protein concentration (1, 2). A similar effect of total protein concentration on the electrophoretic analysis of ovalbumin has been sh...

A study of the Electrophoretic Pattern of the serum Proteins